Flow Cytometers

Within the Cytometry Core, we procure and maintain state-of-the-art instruments for basic and advanced cytometry and single cell analysis applications.

We offer assistance with use of the following instruments: 

BD Accuri C6 Flow Cytometer

  • Lasers
    • 488-nm blue laser
    • 640-nm red laser
  • Detectors
    • 3 fluorescence detectors for the blue laser
    • 1 fluorescence detector for the red laser

Laboratory bench with a BD Accuri C6 flow cytometer connected to a desktop computer showing analysis software, with sample tubes and reagent bottles nearby.

Accuri User Manual

 

CYTEK Northern Lights 1000 Spectral Flow Cytometer

  • Laser
    • 488-nm blue laser
  • Detectors
    • 14 fluorescence detectors covering wavelengths of 498-nm and above
CYTEK NL-1000 laboratory instrument on a bench next to a computer monitor displaying data plots and analysis software.

 

Time-Resolved AcoustoFluidic Flow Cytometer (TRAFFC)

  • Laser
    • 488-nm blue laser
  • Detectors
    • 2 detectors, one for side scatter and one for fluorescenece
  • Fluorescence lifetime enabled acoustically focused flow cytometer
Optical set up of the TRFFC instrument on the optical table with the exposed optical components for laser alignment presented. Including the syringe pump to deliver the sample to the microfluidic chip for measurement and acoustic focusing.

 

Autofluorescence Time-Resolved Flow Cytometer (TRFC)

  • Laser
    • 375-nm violet laser
  • Detectors
    • 2 detectors, one for side scatter and one for fluorescenece
  • Fluorescence lifetime enabled flow cytometer targeted to NAD(P)H autofluorescence for metabolic profiling
The computer screen next to the TRFC instrument on the optical table. The syting pump used to deliver sample to the instrument is also in the image. The TRFC instrument is on an elevated platform and most of it is covered by a black box. However, there are two mirrors are visible, as is the flow cell with a camera in front of the flow cell.

 

Sony MA900 Flow Cytometry Sorter

Please note this instrument is housed and maintained in the Biomecial Research Facility (BRF)

For more information please visit the BRF Website

For Scheduling, Access, and Pricing please contact Alfredo Montoya (alfredom@nmsu.edu) 

The Cytometry Core can provide assistance with data acquisition and analysis once access is approved by the BRF!

  • Lasers
    • 405-nm violet/blue laser
    • 488-nm blue laser
    • 561-nm green laser
    • 638-nm red laser
  • Detectors
    • 12 fluorescence detectors
  • Sorting
    • 4-way sorting available

 MA900 Multi-Application Cell Sorter

 

Complementary Instrumentation

PicoQuant MicroTime 200 Fluorescence Lifetime Imaging Microscope (FLIM)

  • Lasers
    • 375-nm violet laser
    • 405-nm blue laser
  • Detectors
    • Detector 1: Hybrid PMA
      • Sensitive to shorter emission wavelengths
    • Detector 2: SPAD Array
      • Sensitive to higher emission wavelengths

 

Agilent Seahorse HS Mini

  • Real Time Cell Metabolic Analyzer
  • Lable-free microchamber analysis of Oxygen Consumption Rate (OCR) and Extra-Cellular Acidification Rate (ECAR) in real-time for metabolic analysis
Seahorse XF HS Mini Analyzer